Publikationsliste Prof. Dr. Birgitta Wöhrl

  1. J. Drögemüller, C.M. Stegmann, A. Mandal, T. Steiner, B.M. Burmann, M.E. Gottesman, B.M. Wöhrl, P. Rösch, M.C. Wahl, K. Schweimer. 2013.
    An autoinhibited state in the structure of Thermotoga maritima NusG. Structure. 21(3): 365-75.

  2. Schrom, E.-A., R. Moschall, M.J. Hartl, H. Weitner, D. Fecher, J. Langemeier, J. Bohne, B.M. Wöhrl and J. Bodem. 2013.
    U1snRNP-mediated suppression of polyadenylation in conjunction with the RNA structure controls poly (A) site selection in foamy viruses. Retrovirology 10:55

  3. Spannaus, R, A. Schneider, M.J. Hartl, B.M. Wöhrl and J. Bodem. 2013.
    Foamy virus Gag p71-p68 cleavage ist required for template switch of the reverse transcriptase. J. Virol. 87: 7774-7776

  4. Leo, B., K. Schweimer, P. Rösch, M.J. Hartl and B.M. Wöhrl*. 2012.
    The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding. Retrovirol. 9:73

  5. Spannaus R, M.J. Hartl, B.M. Wöhrl, A. Rethwilm and J. Bodem. 2012.
    The prototype foamy virus protease is active independently of the integrase domain. Retrovirol. 9:41

  6. Leo B., M.J. Hartl, K. Schweimer, F. Mayr and B.M. Wöhrl*. 2012.
    Insights into the structure and activity of prototype foamy virus RNase H. Retrovirol. 9:14.

  7. Hartl M.J., B.M. Burmann, S.J. Prasch, C. Schwarzinger, K. Schweimer, B.M. Wöhrl, P. Rösch and S. Schwarzinger. 2012.
    Fast Mapping of Biomolecular Interfaces by Random Spin Labeling (RSL). J. Biomol. Struct. Dyn. 29:793-798.

  8. Hartl M.J., J. Bodem, F. Jochheim, A. Rethwilm, P. Rösch and B.M. Wöhrl*. 2011.
    Regulation of foamy virus protease activity by viral RNA - a novel and unique mechanism among retroviruses. J. Virol. 85: 4462-4469

  9. Hartl, M.J. K.Schweimer, M.H. Reger, S. Schwarzinger, J. Bodem, P. Rösch and B.M. Wöhrl*. 2010.
    Formation of transient dimers by a retroviral protease. Biochem J. 427:197-203

  10. M.J. Hartl, F. Mayr, A. Rethwilm and B.M. Wöhrl*. 2010.
    Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases. Retrovirol. 7:5

  11. Wenzel S., B.M. Martins, P. Rösch, B.M. Wöhrl*. 2010.
    Crystal structure of the human transcription elongation factor DSIF hSpt4 subunit in complex with the hSpt5 dimerization interface.Biochem. J. 425:373-380

  12. Prasch S., M. Jurk, R.S. Washburn, M.E. Gottesman, B.M. Wöhrl, P. Rösch. 2009.
    E. coli NusA recognizes phage λ nut spacer RNA sequences. Nucleic Acids Res. 37:4736-4742

  13. Hartl M.J:, B.M. Wöhrl*, P. Rösch, K. Schweimer. 2008.
    The Solution Structure of the Simian Foamy Virus Protease Reveals a Monomeric Protein. J. Mol. Biol. 381: 141-149

  14. Hartl M.J., B. Kretzschmar, A. Frohn, A. Nowrouzi, A. Rethwilm and B.M. Wöhrl*. 2008.
    AZT resistance of simian foamy virus reverse transcriptase is based on the excision of AZTMP in the presence of ATP. Nucleic Acids Res. 36: 1009-1016

  15. Nageswara Rao J., K. Schweimer, S. Wenzel, B.M. Wöhrl and P. Rösch. 2008.
    NELF-E RRM undergoes major structural changes in flexible protein regions on target RNA binding. Biochemistry 47: 3756-3761

  16. Wenzel, S., K. Schweimer, and P. Rösch and B.M. Wöhrl *. 2008.
    The small hSpt4 subunit of the human transcription elongation factor DSIF is a Zn-finger protein with a/b type topology. Biochem. Biophys. Res.Comm. 370: 414-418

  17. Kretzschmar, B. A. Nowrouzi, M.J. Hartl, K. Gärtner, T. Wiktorowicz, O. Herchenröder, S. Kanzler, W. Rudolph, A. Mergia, B. Wöhrl and A. Rethwilm. 2008.
    AZT-resistant foamy virus. Virology 370: 151-157

  18. Hartl, M.J., B.M. Wöhrl* and K. Schweimer. 2007.
    Sequence-specific 1H, 13C and 15N resonance assignments and secondary structure of a truncated protease from Simian Foamy Virus. Biomol. NMR Assign. 1: 175-177

  19. Berkner, H., J. Engelhorn, D.K. Liscombe, K.Schweimer, B.M. Wöhrl, P.J. Facchini, P. Rösch and I. Matečko. 2007.
    High-yield expression and purification of isotopically labeled Norcoclaurin synthase, a Bet v1 homologous enzyme, from Thalictrum flavum for NMR studies Protein Expr.Purif. 56: 197-204

  20. Nageswara Rao, J., L. Neumann, S. Wenzel, K. Schweimer, P. Rösch and B.M. Wöhrl*. 2006.
    Structural studies on the RNA regognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription. Biochem. J. 400 (3): 449-456

  21. Prasch, S., S. Schwarz, A. Eisenmann, B.M. Wöhrl, K. Schweimer and P. Rösch. 2006.
    Interaction of the intrinsically unstructured phage lambda N Protein with Escherichia coli NusA. Biochemistry 45: 4542-4549

  22. B.M. Wöhrl*, L. Loubière, R. Brundiers, R.S. Goody, D. Klatzmann and M. Konrad. 2005.
    Expression of designed thymidylate kinase variants in human cells to improve AZT phosphorylation and HIV inhibition. J.Gen.Virol. 86: 757-764

  23. Faltz, H., C. Bender, B.M. Wöhrl, K.Vogel-Bachmayr, U. Hübscher, K. Ramadan and J. Liebscher. 2004.
    Double-cyclization of di(alpha-hetarylmethyl)amino esters to optically active bridged N-heterocycles of HIV inhibiting activity. Eur.J.Org.Chem. 2004 (16): 3484-3496

  24. Roda, R.H., M. Balakrishnan, M.N. Hanson, B.M. Wöhrl, S.F. Le Grice, B.P. Roques, R.J. Gorelick and R.A. Bambara. 2003.
    Role of the reverse transcriptase, nucleocapsid protein and template structure on the two-step transfer mechanism in retroviral recombination. J.Biol.Chem. 278: 31536-31546

  25. Rothwell, P.J., S. Berger, O. Kensch, S. Felekyan, M. Antonik , B.M. Wöhrl, T. Restle, R.S. Goody and C.A.M. Seidel. 2003.
    Multi-parameter single molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes. Proc.Natl.Acad.Sci. USA 100: 1655-1660

  26. Werner, S., P. Hindmarsh, M. Napirei, K. Vogel-Bachmayr and B.M. Wöhrl*. 2002.
    Subcellular localization and integration activities of Rous Sarcoma Virus reverse transcriptase. J.Virol. 76: 6205-6212

  27. Werner, S., K. Vogel-Bachmayr, B. Hollinderbäumer and B.M. Wöhrl*. 2001.
    Requirements for minus strand transfer of Rous Sarcoma Virus reverse transcriptase. J.Virol. 75: 10132-10138

  28. Kensch, O., T. Restle, B.M. Wöhrl, R.S. Goody and H.-J. Steinhoff. 2000.
    Temperature-dependent equilibrium between the open and closed conformation of the p66 subunit of the HIV-1 reverse transcriptase revealed by site-directed spin labelling. J.Mol.Biol. 301: 1029-1039

  29. Werner, S. and B.M. Wöhrl*. 2000.
    Homodimeric reverse transcriptases from Rous Sarcoma Virus mutated within the polymerase or RNase H active site of one subunit are active. Eur.J.Biochem. 267: 4740-4744

  30. Werner, S. and B.M. Wöhrl*. 2000.
    Asymmetric subunit organization of Rous Sarcoma Virus reverse transcriptase ab: localization of the polymerase and RNase H active sites in the a subunit. J.Virol. 74: 3245-3252

  31. Werner, S. and B.M. Wöhrl*. 1999.
    Soluble Rous Sarcoma Virus reverse transcriptases a, ab, and b purified from insect cells are processive DNA polymerases that lack an RNase H 3' > 5' directed processing activity. J.Biol.Chem. 274: 26329-26336

  32. B.M. Wöhrl*, R. Krebs, R.S. Goody and T. Restle. 1999.
    Refined model for primer/template binding by HIV-1 reverse transcriptase: pre-steady state kinetic analyses of primer/template binding and nucleotide incorporation events distinguish between different binding modes depending on the nature of the nucleic acid substrate. J.Mol.Biol. 292: 333-344

  33. Souquet, M., T. Restle, R. Krebs, S.F.J. Le Grice, R.S. Goody. and B.M. Wöhrl*. 1998.
    Analysis of the polymerization kinetics of homodimeric EIAV p51/51 reverse transcriptase implies the formation of a polymerase active site identical to heterodimeric EIAV p66/51 reverse transcriptase.Biochemistry 37: 12144-12152

  34. Thrall, S.H., R. Krebs, B.M. Wöhrl, L. Cellai, R.S. Goody and T. Restle. 1998.
    Pre-steady-state characterization of RNA-primed initiation of the transition to a processive DNA-primed polymerization mode.Biochemistry 37: 13349-13358

  35. Krebs, R., U. Immendörfer, S.H. Thrall, B.M. Wöhrl and R.S. Goody. 1997.
    Single step kinetics of HIV-1 reverse transcriptase mutants responsible for virus resistance to nucleoside inhibitors zidovudine and 3-TC.Biochemistry 36: 10292-10300

  36. B.M. Wöhrl*, R. Krebs, S.H. Thrall, S.F.J. Le Grice, A. Scheidig, and R.S. Goody. 1997.
    Kinetic analysis of four HIV-1 reverse transcriptase enzymes mutated in the primer grip: implications for dimerization and DNA synthesis.J. Biol. Chem. 272: 17581-17587

  37. Arts, E.J., S.R. Stetor, X. Li, J.W. Rausch, K.J. Howard, B.Ehresmann, T.W. North, B.M. Wöhrl, R.S. Goody, M.A. Wainberg, and S.F.J. Le Grice. 1996.
    Initiation of (-) strand DNA synthesis from tRNALys,3 on lentiviral RNAs: Implications of specific HIV-1 RNA-tRNALys,3 interactions inhibiting primer utilization by retroviral reverse transcriptases. Proc.Natl.Acad.Sci. USA 93: 10063-10068

  38. Rausch, J.R., E.J. Arts, B.M. Wöhrl, and S.F.J. Le Grice. 1996.
    Involvement of C-terminal structural elements of Equine Infectious Anemia Virus reverse transcriptase in DNA polymerase and ribonuclease H activities. J.Mol. Biol. 257: 500-511

  39. Thrall, S.H., J. Reinstein, B.M. Wöhrl, and R.S. Goody.1996.
    Evaluation of HIV-1 reverse transcriptase primer tRNA binding by fluorescence spectroscopy: specificity and comparison to primer/template binding. Biochemistry 35: 4609-4618

  40. Wehmeier, U.F., B.M. Wöhrl, and J.W. Lengeler. 1995.
    Molecular analysis of the phosphoenolpyruvate-dependent L-sorbose:phosphotransferase system from Klebsiella pneumoniae and of its multidomain structure. Mol.Gen.Genet. 246: 610-618

  41. B.M. Wöhrl, C. Tantillo, E. Arnold, and S.F.J. Le Grice. 1995.
    An expanded biochemical and structural model of translocating human immunodeficiency virus reverse transcriptase.Biochemistry 34: 5343-5350

  42. B.M. Wöhrl, M. Georgiadis, A. Telesnitsky, W. Hendrickson, and S.F.J. Le Grice. 1995.
    Footprinting analysis of replicating murine leukemia virus reverse transcriptase and a polypeptide lacking the C-terminal RNase H domain.Science 267: 96-99

  43. Jacques, P.S., B.M. Wöhrl, K.J. Howard, and S.F.J. Le Grice. 1994.
    Modulation of HIV-1 reverse transcriptase function in "selectively deleted" p66/p51 heterodimers. J. Biol. Chem. 269: 1388-1393.

  44. Jacques, P.S., B.M. Wöhrl, M. Ottmann, J.-L. Darlix, and S.F.J. Le Grice. 1994.
    Mutagenesis within the 'primer grip' of p66 HIV-1 reverse transcriptase implicates Trp229 in template-primer utilization. J. Biol. Chem. 269: 26472-26478.

  45. B.M. Wöhrl, K.J. Howard, P.S. Jacques, and S.F.J. Le Grice. 1994.
    Alternative modes of polymerisation distinguish the subunits of equine infectious anemia virus reverse transcriptase. J. Biol. Chem. 269: 8541-8548.

  46. Volkmann, S., B.M. Wöhrl, M. Tisdale, and K. Moelling. 1993.
    Enzymatic analysis of two HIV-1 reverse transcriptase mutants with mutations in carboxyl-terminal amino acid residues conserved among retroviral ribonucleases H. J. Biol. Chem. 268: 2674-2683.

  47. B.M. Wöhrl, B. Ehresmann, G. Keith, and S.F.J. Le Grice. 1993.
    Nuclease footprinting of human immunodeficiency virus reverse transcriptase/tRNALys-3 complexes. J. Biol. Chem. 268: 13617-13624.

  48. Richter-Cook, N.J., K.J. Howard, N.M. Cirino, B.M. Wöhrl, and S.F.J. Le Grice. 1992.
    Interaction of tRNALys-3 with multiple forms of human immunodeficiency virus reverse transcriptase. J. Biol. Chem. 267: 15952-15957.

  49. B.M. Wöhrl, S. Volkmann, and K. Moelling. 1991.
    Mutations of a conserved residue within HIV-1 ribonuclease H affect its exo-and endonuclease activities. J. Mol. Biol. 220: 801-818.

  50. Lengeler, J.W., F. Titgemeyer, A.P. Vogler, and B.M. Wöhrl. 1990.
    Structure and homologies of carbohydrate:phosphotransferase system (PTS) proteins. Phil. Trans. R. Soc. Lond. B 362: 489-504.

  51. B.M. Wöhrl and J.W. Lengeler. 1990.
    Cloning and physical mapping of the genes for L-sorbose transport and metabolism from Klebsiella pneumoniae. Molec. Microbiol. 4: 1557-1565.

  52. B.M. Wöhrl, G.A. Sprenger, and J.W. Lengeler. 1990.
    Construction of a new catabolic pathway for D-fructose in Escherichia coli K12 using an L-sorbose -specific enzyme from Klebsiella pneumoniae. Arch. Microbiol. 154: 162-167.

  53. B.M. Wöhrl and K. Moelling. 1990.
    Interaction of HIV-1 ribonuclease H with polypurine tract containing RNA-DNA hybrids. Biochemistry 29: 10141-10147.

  54. B.M. Wöhrl, U.F. Wehmeier, and J. W. Lengeler. 1990.
    Positive and negative regulation of the L-sorbose (sor) operon by SorC in Klebsiella pneumoniae. Mol. Gen. Genet. 224: 193-200.